MABE365

Anti-acetyl PARP1 Antibody, clone E4

clone E4, from mouse

• Sinônimo(s): Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: E4, monoclonal
• application: WB
• reatividade de espécies: human
• técnica(s): western blot: suitable
• citations: 1

Propriedades

• fonte biológica: mouse
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: E4, monoclonal
• reatividade de espécies: human
• técnica(s): western blot: suitable
• Isotipo: IgG2bκ
• nº de adesão NCBI: NP_001609
• nº de adesão UniProt: P09874
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: acetylation (not specified)
• Informações sobre genes: human ... PARP1(142)

Descrição

Descrição geral

Poly (ADP-ribose) polymerase 1 (PARP1) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair. As a marker for apoptosis, PARP is cleaved in vitro by many caspases and in vivo by Caspase-3. Acetylation of PARP1 by p300/CREB-binding protein is important in the regulation of NF-kappaB-dependent gene activation through enhanced functional interactions with p300 and the Mediator complex.

Imunogênio

Linear peptide corresponding to acetylated human PARP1, short fragment.

Aplicação

Anti-acetyl PARP1 Antibody, clone E4 is a highly specific mouse monoclonal antibody & that targets PARP & has been tested in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected wild type acetyl-PARP 1, but did not react with four acetylation-deficient PARP1 KQR mutants in WB (Messner, S., et al. (2009). The FASEB Journal. 23:3978-3989).

Qualidade

Evaluated by Western Blotting in acetylated PARP1 fragment (373-529) purified protein lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl PARP1 in 10 µg of acetylated PARP1 fragment (373-529) purified protein lysate.

Descrição-alvo

~ 45 kDa observed. Both proteins we provided were acetylated in vitro by p300 using acetyl-CoA. While the short fragment (bacterially expressed) is very nicely acetylated, the full-length (bacolu system) is only weakly modified. The detection of the full-length protein is thus much more difficult.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Acetylated PARP1 fragment (373-529) purified protein lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable