MABD129

Anti-PRDM1 Antibody, clone 5E7

ascites fluid, clone 5E7, from mouse

• Sinônimo(s): PR domain zinc finger protein 1, BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: ascites fluid
• tipo de produto de anticorpo: primary antibodies
• clone: 5E7, monoclonal
• reatividade de espécies: human
• técnica(s): western blot: suitable
• Isotipo: IgG1
• nº de adesão UniProt: O75626
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PRDM1(639)

Descrição

Descrição geral

PRDM1/Blimp1 is a repressor of beta-interferon gene expression. In the nucleus, PRDM1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, PRDM1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade. The transcription of this gene is increased upon virus infection.

Imunogênio

Purified recombinant fragment of human PRDM1 expressed in E. Coli.

Aplicação

Detect PRDM1 using this mouse monoclonal antibody, Anti-PRDM1 Antibody & clone 5E7 validated for use in western blotting.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Qualidade

Evaluated by Western Blotting in Raji, L1210, and TPH-1 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PRDM1 in Raji, L1210, and TPH-1 cell lysates.

Optimal working dilutions must be determined by end user.

Descrição-alvo

~88 kDa observed. Uncharacterized bands may appear in some lysate(s).

forma física

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Raji, L1210, and TPH-1 cell lysates

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable