MABC1149

Anti-HAF/SART1 Antibody, clone 1-2

clone 1-2, from mouse

• Sinônimo(s): U4/U6.U5 tri-snRNP-associated protein 1, Allergen Hom s 1, HAF, hSART-1, hSnu66, SART-1, Hypoxia-associated factor, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 1-2, monoclonal
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_005137
• nº de adesão UniProt: O43290
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SART1(9092)

Descrição

Descrição geral

U4/U6.U5 tri-snRNP-associated protein 1 (UniProt O43290; also known as Allergen Hom s 1, HAF, hSART-1, hSnu66, Hypoxia-associated factor, SART-1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein) is encoded by the SART1 (also known as HAF) gene (Gene ID 9092) in human. The hypoxia-associated factor (HAF) is an E3 ubiquitin ligase that mediates the ubiquitination and degradation of hypoxia-inducible factor 1α (HIF-1α). HAF also binds to HIF-2α and promotes HIF-2 transactivation instead of promoting HIF-2α degradation. In addition, hypoxia-dependent HAF SUMOylation enhances HAF interaction with HIF-2α and induction of HIF-2 target genes transcription, such as OCT-3/4, SOX2 and NANOG. HAF therefore promotes stem cell characteristics, aggressive tumor growth and invasion by switching cells from HIF-1α- to HIF-2α-dependent signaling. HAF overexpression enhances glioblastoma cells that express both HIF-1α and HIF-2α to initiate tumors as intracranial xenografts in mice. On the other hand, HAF overexpression results in decreased xenograft tumor growth of HT29 colon carcinoma cells that only express HIF-1α.

Especificidade

Clone 1-2 detected HAF shRNA-mediated HAF knockdown in MIA PaCa-2 human pancreatic carcinoma cells.

Imunogênio

GST-tagged recombinant human HAF/SART1 C-terminal fragment.

Aplicação

Detect HAF/SART1 using this mouse monoclonal Anti-HAF/SART1, clone 1-2 Antibody, Cat. No. MABC1149, validated for use in Western Blotting and Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected HAF/SART1 in A431 cells.

Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected HAF/SART1 in 10 µg of Jurkat cell lysate.

Immunocytochemistry Analysis: A representative lot detected a reduced HAF immunoreactivity in MIA PaCa-2 human pancreatic carcinoma cells following HAF shRNA treatment (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunocytochemistry Analysis: A representative lot detected HAF in HeLa and PANC-1 cells (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunocytochemistry Analysis: A representative lot detected an increased HAF immunoreactivity in 786-O human kidney cancer cells transfected to overexpress HAF (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunohistochemistry Analysis: A representative lot detected HAF immunoreactivity in 786-O xenograft (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Research Category
Apoptosis & Cancer

Qualidade

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected HAF/SART1 in 10 µg of HeLa cell lysate.

Descrição-alvo

~110 kDa observed. 90.26 kDa calculated. The larger-than-calculated target band size is consistent with that reported in the literature (Koh, M.Y. et al. (2015). Cancer Res. 75(2):316-329). Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable