MAB1951Z

Anti-Integrin β1 Antibody, clone P4G11, azide free

clone P4G11, Chemicon^®, from mouse

• Sinônimo(s): CD29, MAB1951
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: P4G11, monoclonal
• application: ELISA; FACS; ICC; IHC; IP
• reatividade de espécies: monkey, human, rabbit
• técnica(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• citations: 14

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: P4G11, monoclonal
• reatividade de espécies: monkey, human, rabbit
• fabricante/nome comercial: Chemicon^®
• técnica(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Isotipo: IgG1
• nº de adesão NCBI: NM_002211.2; NM_033666.1; NM_033667.1; NM_033668.1; NM_033669.1; NM_133376.1
• nº de adesão UniProt: P05556
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... ITGB1(3688)

Descrição

Especificidade

Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.

Imunogênio

Human IL-1beta activated endothelial cells

Aplicação

Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.

forma física

Format: Purified

Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.

Armazenamento e estabilidade

Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable