ABE1076

Anti-Misu/NSun2 Antibody

serum, from rabbit

• Sinônimo(s): tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_001180384
• nº de adesão UniProt: Q08J23
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... NSUN2(54888)

Descrição

Descrição geral

Misu/NSun2, also known as tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein (MISU), NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, or tRNA methyltransferase 4 homolog (hTRM4), and encoded by the gene NSUN2/SAKI/TRM4, is a RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Misu/NSun2 methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Functionally Misu/NSun2 acts downstream of Myc to regulate epidermal cell growth and proliferation, and Misu/NSun2-mediated cytosine-5 methylation of vault noncoding RNA helps to determine the processing of them into regulatory small RNAs, and Misu/NSun2 also plays a critical role in gene expression. Interestingly too, Misu/NSun2 is required for proper spindle assembly and chromosome segregation, which is independent of its methyltransferase activity. Misu/NSun2 is localized in the nucleus and nucleolus during rest but during cytokinesis it can be localized in the spindle. Mutations are associated with mental retardation.

Imunogênio

KLH-conjugated linear peptide corresponding to human Misu/NSun2.

Aplicação

Anti-Misu/NSun2 Antibody is an antibody against Misu/NSun2 for use in western blotting, ICC & IHC.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Misu/NSun2 in human keratinocytes (Fry, M., and Watt, F. M. (2006). Curr Biol. 16(10):971-981.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Misu/NSun2 in human epidermis tissue (Fry, M., and Watt, F. M. (2006). Curr Biol. 16(10):971-981.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Qualidade

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Misu/NSun2 in 10 µg of HeLa cell lysate.

Descrição-alvo

~90 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

forma física

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable