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AB2226

Sigma-Aldrich

Anti-MDGA1 Antibody

from rabbit, purified by affinity chromatography

Sinônimo(s):

MAM domain-containing glycosylphosphatidylinositol anchor protein 1, GPI and MAM protein, GPIM, Glycosylphosphatidylinositol-MAM, MAM domain-containing protein 3

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

mouse, rat

reatividade da espécie (prevista por homologia)

human (based on 100% sequence homology), bovine (based on 100% sequence homology)

técnica(s)

western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... MDGA1(266727)

Descrição geral

Studies report that MDGA1 is a layer-specific marker and an area-specific marker, being expressed in layers 2/3 throughout the neocortex, but within the primary somatosensory area (S1), MDGA1 is also uniquely expressed in layers 4 and 6a. Comparisons with other markers, including cadherins, serotonin, cytochrome oxidase, RORß, and COUP-TF1, reveal unique features of patterned expression of MDGA1 within cortex and S1 barrels. Furthermore, during early stages of development, MDGA1 is expressed by Reelin- and Tbr1-positive Cajal–Retzius neurons that originate from multiple sources outside of neocortex and emigrate into it. At even earlier stages, MDGA1 is expressed by the earliest diencephalic and mesencephalic neurons, which appear to migrate from a MDGA1-positive domain of progenitors in the diencephalon and form a "preplate." These findings show that MDGA1 is a unique marker for studies of cortical lamination and area patterning and together with recent reports suggest that MDGA1 has critical functions in forebrain/midbrain development.

Especificidade

This antibody recognizes the MAM domain of MDGA1.

Imunogênio

Epitope: MAM domain
KLH-conjugated linear peptide corresponding to the MAM domain of MDGA1.

Aplicação

Anti-MDGA1 Antibody detects level of MDGA1 & has been published & validated for use in Western Blotting.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Qualidade

Evaluated by Western Blot in untreated and PNGase F treated mouse P7 brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MDGA1 in 5 µg of untreated and PNGase F treated mouse P7 brain tissue lysate. This antibody recognizes glycosolated (>160 kDa) (lane 1) and deglycosolated MDGA1 (lane 2).

Descrição-alvo

~130kDa observed. UniProt describes 2 isoforms produced by alternative splicing at ~106 kDa (Isoform 1), ~107 kDa (Isoform 2). An uncharacterized band may be observed at ~48 kDa in some tissue lysates.

forma física

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Untreated and PNGase F treated mouse P7 brain tissue lysate.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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