71194-M

0.5 M THP Solution

Reducing agent compatible with His•Bind chromatography

• Sinônimo(s): Tris(3-hydroxypropyl)phosphine
• Código UNSPSC: 41106511
• NACRES: NA.77

Propriedades

• Nível de qualidade: 400
• forma: solution
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze
• técnica(s): protein purification: suitable
• aplicação(ões): sample preparation
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Reducing agent compatible with HIS-BIND^® chromatography

THP [Tris(hydroxypropyl)phosphine; C₉H₂₁O₃P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT). Against oxidized glutathione, the reducing capacity of THP (0.232 meq/g) is greater than that of TCEP (0.182 meq/g). THP is also more resistant to air oxidation than DTT and is compatible with immobilized metal affinity chromatography with HIS-BIND^® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM. The common working concentration of THP for many applications is 15 mM. THP is provided as a ready-to-use 0.5 M solution in water.

Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.

Características e benefícios

Compatability with common methods for purifying His-tagged proteins - immobilized metal affinity chromatography with HIS-BIND^® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM

Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)

Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione

Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage

Advertência

Toxicity: Corrosive (L)

Outras notas

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Informações legais

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Danger
• Frases de perigo: H314
• Declarações de precaução: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Classificações de perigo: Eye Dam. 1 - Skin Corr. 1B
• Código de classe de armazenamento: 8B - Non-combustible corrosive hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable