70911

Origami B Competent Cell Set - Novagen

Escherichia coli, rod shaped

• Código UNSPSC: 41106202
• NACRES: NA.81

Propriedades

• product name: Origami B Competent Cell Set - Novagen,
• fonte biológica: Escherichia coli
• Nível de qualidade: 100
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze
• modo de crescimento: adherent or suspension
• morfologia: rod shaped
• técnica(s): microbiological culture: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Genotypes:



Origami B

F^-ompT hsdS_B(r_B^- m_B^-) gal dcm lacY1 ahpC gor522::Tn10 trxB (Kan^R, Tet^R)



Origami B(DE3)

F^-ompT hsdS_B(r_B^- m_B^-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (Kan^R, Tet^R)



Origami B(DE3)pLysS

F^-ompT hsdS_B(r_B^- m_B^-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysS (Cam^R, Kan^R, Tet^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Origami B host strains carry the same trxB/gor mutations as the original Origami strains, except that they are derived from a lacZY mutant of BL21. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner and Origami hosts in one strain background. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, this strain is recommended for use with pET plasmids carrying the ampicillin resistance marker bla.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Componentes

•2 x 0.2 mlOrigami B Competent Cells

•2 x 0.2 mlOrigami B(DE3) Competent Cells

•2 x 0.2 mlOrigami B(DE3)pLysS Competent Cells

•4 x 2 mlSOC Medium

•2 ngTest Plasmid

Advertência

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Informações legais

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2