539568

PP2B, Human, Recombinant, E. coli

• Sinônimo(s): CaN, Protein Phosphatase 2B, Calcineurin
• Código UNSPSC: 12352202

Propriedades

• forma: liquid
• Nível de qualidade: 100
• atividade específica: ≥300,000 U/mg; ≥50,000 units/mL
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Recombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca²⁺/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; Cat. No. 207008) in the presence of calmodulin per min at 30°C, pH 7.4.

forma física

In 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.

Nota de análise

Two distinct bands by SDS-PAGE

Outras notas

Guerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
Mondragon, A., et al. 1997. Biochemistry 36, 4934.
Wei, Q. and Lee, E.Y. 1997. Biochemistry36, 7418.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable