219343

Cathepsin S, Human, Recombinant, E. coli

Cathepsin S, Human, Recombinant, E. coli, is prepared without a tag or fusion protein. A major lysosomal cysteine proteinase with high specific activity.

• Sinônimo(s): Cathepsin S Protein
• Número da licença da enzima: 3.4.22.27 (BRENDA, IUBMB)
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Nível de qualidade: 100
• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥90% (SDS-PAGE)
• forma: liquid
• atividade específica: ≥30,000 mU/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• técnica(s): cell based assay: suitable
• nº de adesão de ID de proteína: AAC37592; AAH02642
• nº de adesão UniProt: P25774
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... CTSS(1520)

Descrição

Descrição geral

Note: 1 mU = 1 milliunit.

Research area: Cell Signaling

Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation.

Aplicação

Cathepsin S, Human, Recombinant, E. coli has been used in cathepsin digestion reactions.

Ações bioquímicas/fisiológicas

Cathepsin S is a cysteine protease that plays a role in degradation of class II complexes in human B lymphocytes. It possesses endoproteolytic activity and aids in processing and presenting antigens to immune cells, thereby influencing the immune response. Cathepsin S is involved in facilitating the degradation of damaged or unwanted proteins within the endo-lysosomal pathway. Dysregulation of Cathepsin S activity leads to the development of various diseases such as arthritis, cancer, and cardiovascular diseases.

Advertência

Toxicity: Harmful (C)

Definição da unidade

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit.

forma física

In 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C).

Outras notas

Liuzzo, J.P., et al. 1999. Mol. Med.5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest.101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest.102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol.244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys.299, 334.
Kirschke, H., et al. 1989. Biochem. J.264, 467.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Warning
• Frases de perigo: H302,H373
• Declarações de precaução: P260 - P264 - P270 - P301 + P312 - P314 - P501
• Classificações de perigo: Acute Tox. 4 Oral - STOT RE 2 Oral
• Órgãos-alvo: Kidney
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1