217669

Anti-CD28 Mouse mAb (ANC28.1/5D10)

liquid, >95% (immunoglobulin, SDS-PAGE), clone ANC28.1/5D10

• Sinônimo(s): Anti-CD28 Antibody, CD28 Detection Antibody, Mouse Anti-CD28
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: ANC28.1/5D10, monoclonal
• Ensaio: >95% (immunoglobulin, SDS-PAGE)
• forma: liquid
• contém: 500 μg/mL gentamycin sulfate as preservative
• reatividade de espécies: human
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Isotipo: IgG1
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CD28(940)

Descrição

Descrição geral

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Protein A purified mouse monoclonal antibody. Recognizes the CD28 protein.

Recognizes CD28 on human T cells and BHK cells transfected with human CD28.

Imunogênio

Human

human CD28 Ig fusion protein and HPB-MLT human tumor cells

Aplicação

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see comments)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstituição

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Outras notas

Kariv, I., et al. 1996. J. Immunol.157, 29.
61st Forum in Immunology. 1995. Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.

Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x10⁵ cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable