17-500M
Catch and Release v2.0 Reversible Immunoprecipitation System
Sinônimo(s):
Catch and release system, Immunoprecipitation kit
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About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NB.22
Produtos recomendados
reatividade da espécie (prevista por homologia)
all
Nível de qualidade
fabricante/nome comercial
Catch And Release
Upstate®
técnica(s)
activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable
Condições de expedição
wet ice
Descrição geral
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Aplicação
Catch and Release v2.0 should not be used with proteins containing a 6-His tag.
Embalagem
50 immunoprecipitations
Componentes
Catch and Release Wash Buffer, 10X (Cat.# 20-210)
Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release Capture Tubes
Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release Capture Tubes
Qualidade
routinely evaluated by immunoprecipitation.
Armazenamento e estabilidade
All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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