16-282

Anti-Phosphotyrosine Antibody, clone 4G10^®, Magnetic Bead Conjugate

clone 4G10®, from mouse

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 4G10®, monoclonal
• reatividade da espécie (prevista por homologia): all
• técnica(s): immunoprecipitation (IP): suitable
• Isotipo: IgG2bκ
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pTyr)
• Informações sobre genes: human ... PID1(55022)

Descrição

Descrição geral

Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Especificidade

This antibody recognizes tyrosine-phosphorylated proteins from all species

Imunogênio

Phosphotyramine-KLH

Aplicação

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Qualidade

100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).

Descrição-alvo

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

forma física

20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.

Format: Purified

Protein G-Sepharose Chromatography

Armazenamento e estabilidade

Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE

Nota de análise

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

Informações legais

4G10 is a registered trademark of Upstate Group, Inc.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2