14-476-M

Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg

Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.

• Sinônimo(s): CDKN7, Cell division protein kinase 7, MO15, STK1, Serine/threonine-protein kinase 1
• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• recombinante: expressed in baculovirus infected Sf21 cells
• forma: liquid
• prazo de validade: 1 yr
• peso molecular: M_w 37.8 kDa (cyclin H); M_w 39.9 kDa (cdk7); M_w 62.8 kDa (MAT1)
• fabricante/nome comercial: Upstate^®
• técnica(s): activity assay: suitable (kinase)
• solubilidade: soluble
• nº de adesão NCBI: NM_001799.2
• nº de adesão UniProt: P50613
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... CDK7(1022)

Descrição

Descrição geral

Research area: APOPTOSIS

Protein Target: CDK7/cyclinH/MAT1 Target Sub-Family: CMGC

Complex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag Product Source: All are expressed by baculovirus in Sf21 insect cells

Cdk-activating kinase (CAK) is a trimeric complex composed of cyclin-dependent kinase 7 (Cdk7), cyclin H, and RING finger protein Methionine Adenosyltransferase 1 (MAT1). Cyclin H activates Cdk7, while MAT1 plays a role in influencing the substrate specificity of the complex.

Ações bioquímicas/fisiológicas

The Cdk7/cyclin H/Mat1 (CAK complex) plays a role in both cell cycle regulation and transcription. Within the complex, Cdk7 functions as the Cdk-activating kinase for cell cycle and as a part of the general transcription factor, Transcription Factor II H (TFIIH), for transcription process. Additionally, it also regulates gene expression by phosphorylating transcription factors such as estrogen receptor-α (ER), p53, retinoid receptors, androgen receptor, and estrogen receptor. Elevated expression levels of Cdk7, cyclin H, and MAT1 have been observed in breast cancer patients. Inhibition of cdk7 is associated with the development of colorectal cancer in humans.

Embalagem

Also available in 250μg size, please inquire for pricing and availability.

Qualidade

routinely evaluated by phosphorylation of cdk7 substrate peptide

forma física

Ni²⁺/NTA-agarose

Armazenamento e estabilidade

1 year at -70°C

Outras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2