07-1585

Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402)

from rabbit, purified by affinity chromatography

• Sinônimo(s): Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• embalagem: antibody small pack of 25 μg
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_001521
• nº de adesão UniProt: Q16665
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HIF1A(3091)

Descrição

Descrição geral

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with HIF-1β to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.

Especificidade

This antibody recognizes HIF-1-alpha at the ODD region.

Imunogênio

Epitope: ODD region

KLH-conjugated linear peptide corresponding to the ODD region of human HIF-1-alpha.

Aplicação

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402) (Rabbit Polyclonal Antibody) validated in WB to detect HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha.

Qualidade

Evaluated by Western Blot in FeCl2 treated HIF-1-alpha recombinant proteins.

Western Blot Analysis: 1 µg/mL of this antibody detected HIF-1-alpha on 10 µg of FeCl2 treated HIF-1-alpha recombinant proteins.

Descrição-alvo

~120 kDa observed using Gal4-tagged recombinant protein. 92 kDa calculated for native protein.

Ligação

Replaces: 04-1006

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
FeCl2 treated HIF-1-alpha recombinant proteins

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.