06-114

Anti-Cathepsin D Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): cathepsin D, cathepsin D (lysosomal aspartyl protease), lysosomal aspartyl peptidase, lysosomal aspartyl protease
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_001900
• nº de adesão UniProt: P07339
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CTSD(1509)

Descrição

Descrição geral

Cathepsin D is synthesized as a 54kDa precursor which is proteolytically processed to an intermediate 48kDa single chain which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and connective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types, especially breast cancer. Cathepsin D is expressed in epithelial cells as well as in macrophages. Immunoreactivity of cancer cells and tissue infiltrating macrophages must be recorded separately.

Especificidade

84% sequence homology to mouse and rat.

This antibody recognizes Cathepsin D.

Imunogênio

Epitope: Heavy Chain

GST-tagged recombinant protein corresponding to human Cathepsin D at the heavy chain.

Aplicação

Anti-Cathepsin D Antibody is an antibody against Cathepsin D for use in WB & IC.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cathepsin D in A431 and HeLa cell lystes.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Apoptosis - Additional

Qualidade

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Cathepsin D on 10 µg of A431 cell lysate.

Descrição-alvo

Observed molecular weight at ~58 kDa Protein is glycosylated and may run slightly higher than predicted.
Predicted molecular weight of the active form is ~46 kDa and ~ 54 kDa for the precursor form.

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
A431 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable