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Merck
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Documentos Principais

04-928

Sigma-Aldrich

Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

culture supernatant, clone A3S, Upstate®

Sinônimo(s):

H3, Histone H3

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

culture supernatant

tipo de produto de anticorpo

primary antibodies

clone

A3S, monoclonal

reatividade de espécies

human, rat, Saccharomyces cerevisiae, mouse, chicken

fabricante/nome comercial

Upstate®

técnica(s)

ChIP: suitable
western blot: suitable

Isotipo

IgG

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... H3F3B(3021)

Descrição geral

An antibody that recognizes the C-terminus of histone H3 and is useful for identifying total H3 independant of post-translational modifications.

Especificidade

Broad species cross-reactivity expected due to sequence homology
Recognizes Histone H3.

Imunogênio

KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3

Aplicação

Anti-Histone H3 Antibody, CT, pan, clone A3S is a Rabbit Monoclonal Antibody for detection of Histone H3 also known as Histone H3 & has been validated in ChIP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Chromatin Immunoprecipitation:
2 μL of of a previous lot of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.

Qualidade

Routinely evaluated by immunoblot analysis.

Descrição-alvo

17 kDa

Ligação

Replaces: 05-928

forma física

Cultured supernantant in 0.05% sodium azide

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Marcel Donnet et al.
Journal of colloid and interface science, 340(2), 218-224 (2009-10-10)
Thermodynamic solubility calculations are normally only related to thermodynamic equilibria in solution. In this paper, we extend the use of such solubility calculations to help elucidate possible precipitation reaction pathways during the entire reaction. We also estimate the interfacial energy
Huiyuan Zhang et al.
Nature communications, 5, 5798-5798 (2014-12-17)
The transcriptional regulator STAT3 curbs pro-inflammatory cytokine production mediated by NF-κB signalling in innate immune cells, yet the mechanism by which this occurs has been unclear. Here we identify STAT3 as a pivotal negative regulator of Ubc13, an E2 ubiquitin-conjugating
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
Briggs, S D, et al.
Genes & Development, 15, 3286-3295 (2001)
Jessica Kain et al.
Molecular metabolism, 53, 101291-101291 (2021-07-12)
Type II nuclear hormone receptors, including farnesoid X receptors (FXR), liver X receptors (LXR), and peroxisome proliferator-activated receptors (PPAR), which serve as drug targets for metabolic diseases, are permanently positioned in the nucleus and thought to be bound to DNA
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.
Xiao, Tiaojiang, et al.
Genes & Development, 17, 654-663 (2003)

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