900473
Gold nanoparticles
10 nm, NHS ester functionalized, conjugation kit
Sinônimo(s):
NHS-activated gold nanoparticles
About This Item
Produtos recomendados
forma
nanoparticles
prazo de validade
12 mo. at -20 °C (If stored unopened and as specified, are stable for at least 12 months)
embalagem
pkg of 1 ea
condição de armazenamento
protect from light
tamanho de partícula
10 nm
λmax
515-520 nm
grupo funcional
NHS ester
temperatura de armazenamento
−20°C
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Descrição geral
•3 vials of 10 nm NHS-Activated Gold Nanoparticles (lyophilized)
•Protein Resuspension Buffer - 1.5ml
•Reaction Buffer - 1.5ml
•Quencher Solution - 1.5ml
•Reaction protocol
The gold nanoparticles in this kit are supplied as a lyophilized (freeze-dried) mixture. The conjugation reaction is initiated by simply reconstituting the freeze-dried nanoparticles with the protein of interest, which attaches to the nanoparticle surface via lysine residues.
The gold conjugation procedure has a quick hands-on time of around 10 minutes, and the conjugate is fully ready for use within 3 hr. Researchers only need to pipette the biomolecule into a vial containing the nanoparticles, quench the reaction, and perform product washing through microcentrifugation.
The provided protocol is optimized for highly efficient covalent conjugation, resulting in stable gold nanoparticle conjugates. Optional protocol steps involve additional materials, such as 10% aqueous BSA (126615) and Tris saline resuspension buffer (PPB002), which are sold separately.
Aplicação
Different sizes of gold nanoparticles perform better in specific applications. For lateral flow and flow cytometry, we recommend using gold nanoparticles with diameters between 20 nm and 100 nm. For immunoblotting or immuno-electron microscopy, the optimal range is 5 nm to 40 nm. For bioassays utilizing dynamic light scattering, gold nanoparticles with diameters ranging from 60 nm to 100 nm are suggested.
Características e benefícios
•Fast and convenient conjugation reaction with no need for pre-activation.
•A spacer between the gold nanoparticle surface and the conjugated ligand minimizes the impact on the tertiary protein structure. This reduces the likelihood of poor performing conjugates, a common issue encountered with conjugates prepared by passive adsorption.
•Gold surface coating is precisely engineered to minimize non-specific protein binding in biological assays.
Informações legais
Código de classe de armazenamento
10 - Combustible liquids
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Artigos
Drs. Fernandes and Baptista explore the synthesis and functionalization of gold nanoparticles and their use in molecular diagnostics, focusing on lateral flow assays. They further discuss the regulations and standards of these materials, as well as the future outlook and challenges.
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