SRP0364

mTOR/Raptor/MLST8 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥50% (SDS-PAGE)

• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥50% (SDS-PAGE)
• form: aqueous solution
• mol wt: 137 kDa (mTOR); 150 kDa (Raptor); 37 kDa (MLST8)
• packaging: pkg of 10 μg
• manufacturer/tradename: Sigma-Aldrich
• technique(s): cell based assay: suitable
• solubility: water: soluble
• NCBI accession no.: NM_004958 (mTOR); NM_020761 (Raptor); NM_022372 (MLST8)
• application(s): life science and biopharma
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MLST8(64223) , MTOR(2475) , RPTOR(57521)

Description

General description

This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human mTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.

Application

Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.

Biochem/physiol Actions

Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. mTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory-associated protein of mTOR complex 1 (RAPTOR) has an important role in modulating cell growth and energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of the mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable