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SHPMLIBR

Sigma-Aldrich

MISSION® LentiPlex® Complete Mouse Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

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About This Item

UNSPSC Code:
41105904
NACRES:
NA.51

Quality Level

200

product line

MISSION®

concentration

≥5x108 VP/ml (via p24 assay)

technique(s)

capture ELISA: 5 x 108 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

Related Categories

General description

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma′s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Complete Mouse shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 110,000 shRNA constructs from the TRC collection targeting 21,000+ mouse genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into fifteen subpools of approximately 5,500-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

For more information including sample data and uses, please visit our LentiPlex Technical Detail Page.

Other Notes

The MISSION LentiPlex Mouse Pooled shRNA Libraries consist of Catalog Numbers SHPM01, SHPM15, SHPM2, and SHPMLIBR. Please refer to Table 1 for approximate number of shRNA clone coverage, targeted genes, and subpools for each library.

Amplification and sequencing primers are also provided for downstream hit identification.

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

  • Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
  • Comprehensive, reproducible results from pooled shRNA screens
  • Statistically robust and information-rich data

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

Recommended products

Browse additional MISSION shRNA Gene Family Sets in our MISSION shRNA portal

Legal Information

Lentiviral License Agreement
LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Safe Lentivirus Handling

Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.

Successful Transduction Using Lentivirus

Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts.

Protocols

CRISPR Lentiviral Screening: Antibiotic Selection

You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

FACS Titration of Lentivirus Protocol

FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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