L9017
Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder
Synonym(s):
Phaseolus vulgaris agglutinin, PHA
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
Phaseolus vulgaris
product line
BioXtra
form
lyophilized powder
potency
≤17 μg per mL agglutination activity
purified by
affinity chromatography
impurities
salt, essentially free
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
General description
Lectins are carbohydrate-binding proteins, that contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.
Application
Lectin from Phaseolus vulgaris (red kidney bean) has been used:
- for phytohemagglutinin (PHA) swelling assay
- for lymphocyte activation assay
- to evaluate cell-mediated immune response
Biochem/physiol Actions
Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It has anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin is associated with toxic allergens and hemagglutinins. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Quality
Mitogenic activity at ≤10 μg per mL.
Analysis Note
Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
The Lectins: Properties, Functions, and Applications in Biology and Medicine (2012)
Chickadees faced with unpredictable food increase fat reserves but certain components of their immune function decline
Cornelius EA, et al.
Physiological and Biochemical Zoology : Pbz, 90(2), 190-200 (2017)
Effect of propolis and zinc methionine supplementation on improvement of productive, reproductive and immunity performance of local developed in sash strain under egyptian summer conditions.
Habeib AA, et al.
Egyptian Poultry Science Journal, 39(1), 253-273 (2019)
Second case of HOIP deficiency expands clinical features and defines inflammatory transcriptome regulated by LUBAC
Oda H, et al.
Frontiers in Immunology, 10, 479-479 (2019)
Jon S Beadell et al.
PloS one, 2(9), e896-e896 (2007-09-20)
Dramatic declines of native Hawaiian avifauna due to the human-mediated emergence of avian malaria and pox prompted an examination of whether island taxa share a common altered immunological signature, potentially driven by reduced genetic diversity and reduced exposure to parasites.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service