L8754
Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, lyophilized powder
Synonym(s):
Phaseolus vulgaris agglutinin, PHA
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About This Item
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biological source
Phaseolus vulgaris
form
lyophilized powder
potency
≤62.5 μg per mL agglutination activity
≤10 μg/mL mitogenic activity
impurities
salt, free
storage temp.
2-8°C
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General description
PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.
Application
It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.
Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.
Biochem/physiol Actions
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Analysis Note
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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