I5278
Insulin-Like Growth Factor Binding Protein-3 human
recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, ≥95% (SDS-PAGE)
Synonym(s):
IGF Binding Protein-3, IGFBP-3
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About This Item
Recommended Products
biological source
human
Quality Level
recombinant
expressed in NSO cells
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
0.01-0.25 μg/mL
mol wt
29 kDa
packaging
pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IGFBP3(3486)
Biochem/physiol Actions
Human IGFBP-3 is the major insulin-like growth factor (IGF) binding protein in plasma, where it exists in a ternary complex with IGF-I or IGF-II and the acid-labile subunit (ALS).
IGFBPs (insulin like growth factor binding proteins) regulate bioavailability of IGFs (insulin like growth factors) in cells. Binding between IGFs and IGFBPs reduces the availability of the IGFs. However, IGFBPs also concentrate IGFs in specific areas to potentiate their effects at right time. IGFBP3 binds to IGF-1. It is a tumor suppressor and is downregulated in many cancers. In serum, IGFs, IGFBP3 and the acid labile subunit (ALS) exist together as a complex. This complex is important for increasing the half life of serum IGFs.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA.
Analysis Note
The biological acitivity is measured as the ability to inhibit the biological activity of 14 ng/ml recombinant human IGF-I on MCF-7 cells.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Spagnoli, A. and Rosenfeld, R.G.
Curr. Op. Endocrinology and Diabetes, 4, 1-1 (1997)
Chuan-Wei Yang et al.
Journal of the American Medical Directors Association, 16(5), 365-370 (2014-12-31)
Previous studies have demonstrated the polymorphisms of insulinlike growth factor-1 (IGF-1) and its binding protein-3 (IGFBP3) genes could affect the circulating IGF-1 level. Moreover, the serum IGF-1 level was correlated with muscle size. This study aimed to explore the effect
Kelley, K.M., et al.
The International Journal of Biochemistry & Cell Biology, 28, 619-637 (1995)
Min Sun Kim et al.
Molecular and cellular biochemistry, 403(1-2), 107-113 (2015-02-11)
Nuclear factor-kappaB (NF-κB) is a transcription factor that is activated in various neoplasms, including gastric cancer. Insulin-like growth factor binding protein-3 (IGFBP-3) is a potent tumor suppressor and is significantly suppressed in a variety of cancers. Although IGFBP-3 has been
M L Cubbage et al.
The Journal of biological chemistry, 265(21), 12642-12649 (1990-07-25)
Insulin-like growth factor binding protein-3 (IGFBP-3) can modulate the mitogenic and metabolic effects of the insulin-like growth factors (IGFs). IGFBP-3 protein levels are developmentally regulated and influenced by a number of hormonal stimuli both in vitro and in vivo. As
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