G7277
Anti-Rabbit IgG (whole molecule)−Gold antibody produced in goat
affinity isolated antibody, aqueous glycerol suspension, 5 nm (colloidal gold)
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About This Item
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biological source
goat
Quality Level
conjugate
gold conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
aqueous glycerol suspension
availability
not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)
technique(s)
dot blot: suitable
western blot: suitable
particle size
5 nm (colloidal gold)
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
General description
Goat polyclonal anti-Rabbit IgG (whole molecule)−Gold antibody binds all rabbit IgGs. It has no observed reactivity with human serum proteins.
Immunoglobulins (Igs) have two heavy (H) and two light (L) chains, held together by disulphide linkages. They belong to the immunoglobulin super-family. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The light chain has one variable and constant region. IgGs are subclassified into IgG1, IgG2, IgG3 and IgG4. The IgG gene is localised on rabbit chromosome 20. Gene conversion events are observed in rabbit IgG gene.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Immunogold labeling is a technique whereing antibodies, especially secondary antibodies, are labeled with colloidal gold. These gold conjugated antibodies generate electron dense particles that are visable using electron microscopy.
Immunogold labeling is a technique whereing antibodies, especially secondary antibodies, are labeled with colloidal gold. These gold conjugated antibodies generate electron dense particles that are visable using electron microscopy.
Application
Anti-Rabbit IgG (whole molecule)−Gold antibody produced in goat has been used in electron microscopic analysis of exosomes in glioma cells, clathrin proteins and retinal tissues.
Goat polyclonal anti-Rabbit IgG (whole molecule)−Gold antibody may be used as a primary or secondary antibody that enables the labeling of rabbit IgG for detection by electron microscopy.
Biochem/physiol Actions
Digestion of IgG by papain results in generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). Fc regions of IgG antibody have enormous therapeutic potential and are exploited for the development of therapeutic antibodies. IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.
Other Notes
Antibody adsorbed with human serum proteins.
Physical form
Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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