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Sigma-Aldrich

AccuTaq™ LA 10× Buffer

10X Buffer for long and accurate PCR

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About This Item

UNSPSC Code:
41106306

grade

for molecular biology

Quality Level

200

form

liquid

packaging

vial of 0.5 mL

concentration

10 ×

technique(s)

PCR: suitable

storage temp.

−20°C

General description

AccuTaq™ LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.

Application

AccuTaq™ LA 10× Buffer has been used:

  • in PCR reaction mixture for the amplification of human BRCA1 gene.
  • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum
  • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)

Legal Information

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Baozhong Meng et al.
The Journal of general virology, 86(Pt 5), 1555-1560 (2005-04-16)
Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have
Jeong-Yun Choi et al.
The Journal of biological chemistry, 286(36), 31180-31193 (2011-07-26)
The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts. Both mRNAs and proteins of all four DNA
Vandana Jaiswal et al.
PloS one, 10(6), e0129400-e0129400 (2015-06-16)
TaGW2 is an orthologue of rice gene OsGW2, which encodes E3 RING ubiquitin ligase and controls the grain size in rice. In wheat, three copies of TaGW2 have been identified and mapped on wheat homoeologous group 6 viz. TaGW2-6A, TaGW2-6B
Eva-Maria Ladenburger et al.
Molecular and cellular biology, 29(13), 3605-3622 (2009-04-22)
A database search of the Paramecium genome reveals 34 genes related to Ca(2+)-release channels of the inositol-1,4,5-trisphosphate (IP(3)) or ryanodine receptor type (IP(3)R, RyR). Phylogenetic analyses show that these Ca(2+) release channels (CRCs) can be subdivided into six groups (Paramecium
David T Hughes et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(21), 9831-9836 (2010-05-12)
The mammalian gastrointestinal (GI) tract is colonized by a complex consortium of bacterial species. Bacteria engage in chemical signaling to coordinate population-wide behavior. However, it is unclear if chemical sensing plays a role in establishing mammalian host-bacterial commensal relationships. Enterohemorrhagic
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Protocols

Long and Accurate PCR Amplification of DNA with RedAccuTaq® (D4812)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA

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