D5809

JumpStart^™ AccuTaq^™ LA DNA Polymerase

Hot-start high fidelity Taq enzyme, 10X buffer included

• Synonym(s): JumpStart^® AccuTaq^™ LA DNA Polymerase
• UNSPSC Code: 41106314
• NACRES: NA.55

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 125 reactions; sufficient for 500 reactions
• feature: Long & Accurate PCR; dNTPs included: no; hotstart
• concentration: 2.5 unit/μL
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• suitability: suitable for PCR
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

JumpStart^™ AccuTaq^™ LA DNA Polymerase is a combination of Sigma′s high-fidelity blend, AccuTaq long and accurate (LA) DNA Polymerase, and the Taq neutralizing JumpStart Taq antibody. This specially formulated enzyme is the best choice for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot-start mechanism provides increased specificity and higher target yield in comparison to standard amplification.

Application

JumpStart^™ AccuTaq^™ LA DNA Polymerase has been used:

• in polymerase chain reaction (PCR) for the amplification of soil DNA samples
• in accurate long range PCR of histone H2A.Z in bacterial artificial chromosome (BACs)
• as a component of the PCR reaction mix for amplification of viral DNA through conventional PCR

Features and Benefits

• JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• Up to 6.5× greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR
• Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
• Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods

Packaging

Supplied with 10× reaction buffer.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Jacobs is a registered trademark of Jacobs

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves