- Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol.
Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol.
FEBS letters (2015-04-12)
Suguru Komenoi, Fumika Takemura, Hiromichi Sakai, Fumio Sakane
PMID25862496
RÉSUMÉ
Diacylglycerol kinase (DGK) η plays important roles in various patho-physiological events such as oncogenesis. In this study, we performed an enzymological characterization of DGKη splice variant 1 (DGKη1). The Km value for diacylglycerol was 0.14 mol%. Intriguingly, the Km value of DGKη1 for diacylglycerol was at least 9-fold lower than those of other DGK isozymes including DGKα, indicating that DGKη1 is a high affinity isozyme for diacylglycerol. Therefore, DGKη1 is a unique DGK isozyme, which may function at particular membrane sites where only low concentrations of diacylglycerol are supplied.
MATÉRIAUX
Référence du produit
Marque
Description du produit
Sigma-Aldrich
Chlorure de sodium, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
Chlorure de sodium solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
Chlorure de sodium, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Chlorure de sodium, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%