- Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells.
Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells.
Chemical communications (Cambridge, England) (2013-11-28)
Aoi Teraoka, Kanako Murakoshi, Kyoko Fukamauchi, Akinobu Z Suzuki, Soichiro Watanabe, Toshiaki Furuta
PMID24282826
RÉSUMÉ
A facile and useful method for preparing caged DNAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.
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