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52797-U

Supelco

HybridSPE®-PL solid phase extraction (SPE) Cartridge

Bed wt. 100 mg, volume 3 mL, pk of 54 ×

Synonyme(s) :

HybridSPE®-Phospholipid

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About This Item

Code UNSPSC :
41115712
Nomenclature NACRES :
NB.21

product name

HybridSPE-PL Cartridge, Bed wt. 100 mg, volume 3 mL, pk of 54 ×

Matériaux

PE frit (20/40 μm)
polypropylene hardware

Niveau de qualité

100

Description

Cartridge

Composition

Bed wt., 100 mg

Conditionnement

pk of 54 ×

Technique(s)

solid phase extraction (SPE): suitable

Volume

3 mL

Groupe de la matrice active

zirconia-based phase

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Description générale

HybridSPE-Phospholipid technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is loaded on the HybridSPE-Phospholipid 96-well plate or cartridge which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids. The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE-Phospholipid stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS-MS analysis.

The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.

Caractéristiques et avantages

  • Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
  • Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
  • 2-3 step generic procedure
  • Minimal to no method development
  • Available in 96-well and 1 mL cartridge dimensions

Informations légales

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
16867002
16867001
16036802
16036801
15737901

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

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