ZRB2565

Anti-ROR2 clone XBR2-401 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Tyrosine-protein kinase transmembrane receptor ROR2;EC:2.7.10.1, Neurotrophic tyrosine kinase, receptor-related 2)
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: XBR2-401, recombinant monoclonal
• Description: XBR2-401 Clone
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 104.76 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): ELISA: suitable; affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable
• Isotype: IgG
• Séquence de l'épitope: Extracellular domain
• Numéro d'accès Protein ID: NP_004551.2
• Numéro d'accès UniProt: Q01974
• Autre catégorie plus écologique: , Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ROR2(4920)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone XBR2-401 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Tyrosine-protein kinase transmembrane receptor ROR2. It targets an epitope within the Kringle domain from the extracellular region.

Immunogène

From naïve rabbit antibody library (as described by Peng, H., et al. (2017). J. Mol. Biol. 429(19): 2954 2973).

Application

Quality Control Testing

Evaluated by Immunocytochemistry in T47D cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected ROR2 in T47D cells.

Tested Applications

Flow Cytometry Analysis: 1 µg from a representative lot detected ROR2 in one million T47D cells.

Western Blotting Analysis: A representative lot detected ROR2 in Western Blotting applications (Peng, H., et. al. (2017). J Mol Biol. 429 (19); 2954-2973).

Affinity Binding Assay: A representative lot of this antibody bound recombinant fragment of Human ROR2 with a KD of 2.5 x 10-6 in an affinity binding assay.

Flow Cytometry Analysis: A representative lot detected ROR2 in Flow Cytometry applications (Peng, H., et. al. (2017). J Mol Biol. 429(19); 2954-2973).

ELISA Analysis: A representative lot detected ROR2 in ELISA applications (Peng, H., et. al. (2017). J. Mol Biol. 429(19); 2954-2973).

Enzyme Immunoassay Analysis: Various dilutions of this antibody detected recombinant fragment of Human ROR2.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Tyrosine-protein kinase transmembrane receptor ROR2 (UniProt: Q01974; also known as EC:2.7.10.1, Neurotrophic tyrosine kinase, receptor-related 2) is encoded by the ROR2 (also known as NTRKR2) gene (Gene ID: 4920) in human. ROR2 is a homodimeric, single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 34-403), a transmembrane domain (aa 404-424), and a cytoplasmic domain (aa 425-943). Its protein kinase domain is localized in amino acids 473-746. ROR2 is expressed at high levels during embryonic development and its expression levels decrease significantly around day 16 and adults display much reduced levels. ROR2 is involved in the early formation of chondrocytes and may be involved in cartilage and growth plate development. It phosphorylates tyrosine 3-Monooxygenase (YWHAB), leading to induction of osteogenesis and bone formation. It displays very little tyrosine kinase activity in vitro. Higher expression of ROR2 has been reported in several cancers, including solid malignancies, such as renal cell adenocarcinoma, breast cancer, and hematologic malignancies, such as multiple myeloma. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goydel, RS., et al. (2020). J. Biol. Chem. 295(18); 5995-6006; Peng, H., et al. (2017). J Mol. Biol. 429(19); 2954-2973; Liu, Y., et al. (2007). Mol. Endocrinol. 21(12); 3050-3061).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable