ZRB1214
Anti-MYH1 Antibody, clone 1E15 , ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonyme(s) :
MyHC-2x, MyHC-IIx/d, Myosin heavy chain, Myosin heavy chain 2x, Myosin heavy chain IIx/d, Myosin-1, skeletal muscle adult 1
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About This Item
Code UNSPSC :
12352203
Source biologique
rabbit (recombinant)
Niveau de qualité
Produit recombinant
expressed in HEK 293 cells
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
1E15, recombinant monoclonal
Gamme de produits
ZooMAb® learn more
Forme
lyophilized
Poids mol.
calculated mol wt 223.15 kDa
observed mol wt ~225
Espèces réactives
human, mouse
Conditionnement
antibody small pack of 25 μL
Caractéristiques du produit alternatif plus écologique
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
Validation améliorée
recombinant expression
Learn more about Antibody Enhanced Validation
Technique(s)
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Isotype
IgG
Numéro d'accès UniProt
Autre catégorie plus écologique
, Aligned
Conditions d'expédition
ambient
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MYH1(4619)
Description générale
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Spécificité
Clone 1E15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Myosin Heavy Chain 1. it targets an epitope within 18 amino acids from the N-terminal region.
Immunogène
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Myosin Heavy Chain 1.
Application
Anti-MYH1, clone 1E15 ZooMAb, Cat. No. ZRB1214, is a highly specific recombinant rabbit monoclonal antibody that detects Myosin heavy chain 1 (MYH1) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin) and, Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MYH1 in C2C12 cell lysate.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MYH1 in human skeletal muscle tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYH1 in human skeletal muscle and mouse skeletal muscle tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MYH1 in human skeletal muscle tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYH1 in human skeletal muscle and mouse skeletal muscle tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.
Description de la cible
Myosin-1 (UniProt: P12882; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, Myosin heavy chain, skeletal muscle adult 1) is encoded by the MYH1 gene (Gene ID: 4619) in human. Myosin is a major contractile protein that converts chemical energy into mechanical energy through the hydrolysis of ATP. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-1 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-785), and an IQ domain (aa 788-817). It also contains 2 actin binding regions (aa 662-684 and 764-778). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Forme physique
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Stockage et stabilité
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Informations légales
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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