ZMS1064
Anti-Dynein Antibody, clone 74.1 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
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About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
mouse
Niveau de qualité
Produit recombinant
expressed in HEK 293 cells
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
74.1, recombinant monoclonal
monoclonal
Gamme de produits
ZooMAb® learn more
Forme
lyophilized
Poids mol.
calculated mol wt ~68.56 kDa
observed mol wt ~78 kDa
Espèces réactives
rat, human, mouse
Réactivité de l'espèce (prédite par homologie)
Xenopus, bovine, sheep, fish, Drosophila
Conditionnement
antibody small pack of 25 μL
Caractéristiques du produit alternatif plus écologique
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
Validation améliorée
recombinant expression
Learn more about Antibody Enhanced Validation
Technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
Isotype
IgG2b
Numéro d'accès UniProt
Autre catégorie plus écologique
Conditions d'expédition
ambient
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Description générale
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Spécificité
Clone 74.1 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects cytoplasmic dynein.
Immunogène
Purified native bovine brain cytoplasmic dynein.
Application
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Dynein in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Dynein in A431 and PC12 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Dynein in HeLa, HepG2, and NIH 3T3 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Dynein in one million HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Dynein in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Dynein in A431 and PC12 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Dynein in HeLa, HepG2, and NIH 3T3 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Dynein in one million HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Dynein, clone 74.1 ZooMAb®, Cat. No. ZMS1064, is a recombinant Mouse monoclonal antibody that specifically targets Dynein and is tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
Description de la cible
Cytoplasmic dynein 1 intermediate chain 1 (UniProt: Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) is encoded by the DYNC1I1 gene (Gene ID: 613724) in bovine. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 It is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. It is a multi-subunit complex of 1.5 MDa composed of a homodimer of heavy chains, two intermediate chains, light-intermediate chains, and light chains. The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. Cytoplasmic dynein 1 intermediate chain 1 acts as one of the several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are involved in linking dynein to cargos and to adapter proteins that regulate dynein function. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Forme physique
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Stockage et stabilité
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Informations légales
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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