ZMS1039

Anti-α-Tubulin Antibody, clone DM1A ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Anti-B-ALPHA-1, Anti-TUBA3
• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: DM1A, recombinant monoclonal; monoclonal
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt ~50.14 kDa; observed mol wt ~48 kDa
• Espèces réactives: human, mouse, rat
• Réactivité de l'espèce (prédite par homologie): porcine, guinea pig, avian, bovine
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: Q71U36
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone DM1A is a ZooMAb^® mouse recombinant monoclonal antibody that detects a-Tubulin in multiple species. It targets an epitope within 24 amino acids from the C-terminal region.

Immunogène

Full-length a-Tubulin from human brain.

Application

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a-Tubulin in MCF-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected a-Tubulin in NIH3T3 and PC12 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected a-Tubulin in human kidney and human testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected a-Tubulin in HeLa, A431, HepG2, and NIH 3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-a-Tubulin, clone DM1A ZooMAb^®, Cat. No. ZMS1039, is a recombinant Mouse monoclonal antibody that specifically targets a-Tubulin and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Description de la cible

Tubulin alpha-1A chain (UniProt: Q71U36; also known as Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain) is encoded by the TUBA1A (also known as TUBA3) gene (Gene ID: 7846) in human. Tubulin is the major constituent of microtubules (MTs). MTs are cytoskeletal filaments composed of  a /β-tubulin heterodimers. Tubulin dimers constantly polymerize and depolymerize, and MTs can undergo rapid cycles of assembly and disassembly. In the presence of Mg2+ and GTP, a and β tubulins join in an end-to-end manner to form protofilaments with alternating a and β subunits. Tubulin binds two moles of GTP, one at an exchangeable site on the ß-chain and one at a non-exchangeable site on the a-chain. GTP bound to a-tubulin is hydrolyzed to GDP during or immediately after polymerization. This weakens the binding affinity of tubulin for adjacent molecules and favors depolymerization that contributes to the dynamic behavior of MTs. MTs also undergo treadmilling, in which tubulin molecules bound to GDP are continually lost from the minus end and are replaced by the addition of GTP-bound tubulin molecules to the plus end of the same MT. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins and motor proteins. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1