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Sigma-Aldrich

Extract-N-Amp Plant PCR Kit

sufficient for 100 extractions, sufficient for 500 amplifications

Synonyme(s) :

Plant direct PCR kit

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About This Item

Code UNSPSC :
41106303
Nomenclature NACRES :
NA.55

Utilisation

sufficient for 100 extractions
sufficient for 500 amplifications
sufficient for 500 reactions

Niveau de qualité

Caractéristiques

dNTPs included
hotstart

Technique(s)

PCR: suitable

Couleur

colorless

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Description générale

The Extract-N-Amp Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.
The PCR reaction mix facilitates amplification directly from the extract by using an antibody-based hot-start DNA polymerase for specific amplification.

Application

Extract-N-Amp Plant PCR Kit has been used to extract plant genomic DNA and in polymerase chain reaction (PCR).

Extract-N-Amp Plant PCR Kit is suitable for use in genotyping.

It has been used forDNA extraction from fresh mushrooms.

Caractéristiques et avantages

  • Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
  • A specially formulated PCR ReadyMix is provided for use with the extracted DNA
  • The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
  • Suitable for high-throughput plant genetic analysis.
  • Lengthy enzymatic digestions are not required, saving time and effort.
  • The extracted DNA remains stable at 4 °C for atleast 6 months.

Composants

Extract-N-Amp Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)
Extraction Solution- E7526-12 mL

Dilution Solution- D5688-12mL

Extract-N-Amp PCR ReadyMix. This 2X PCR reaction mix contains buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody.- E3004- 5 x 1.2 ml

Collection tubes

Autres remarques

The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Informations légales

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Noa Eliahu et al.
Eukaryotic cell, 6(3), 421-429 (2007-01-24)
The maize pathogen Cochliobolus heterostrophus requires two mitogen-activated protein kinases (MAPKs), Chk1 and Mps1, to produce normal pigmentation. Young colonies of mps1 and chk1 deletion mutants have a white and autolytic appearance, which was partially rescued by a hyperosmotic environment.
Sophie Lev et al.
Molecular plant-microbe interactions : MPMI, 22(9), 1093-1103 (2009-08-07)
Phosphorylated mitogen-activated protein kinases (MAPK) transmit signals by activation of their targets. The extent of signal transduction could depend on MAPK phosphorylation level, concentration, and subcellular localization. The pathogenicity MAPK Chk1 of the fungal corn pathogen Cochliobolus heterostrophus is required
Len N Gillman et al.
Journal of evolutionary biology, 23(6), 1327-1330 (2010-05-12)
A faster rate of nuclear DNA evolution has recently been found for plants occupying warmer low latitudes relative to those in cooler high latitudes. That earlier study by our research group compared substitution rates within the variable internal transcribed spacer
S A Weller et al.
Journal of microbiological methods, 70(2), 379-383 (2007-06-26)
Real-time (TaqMan) PCR assays were developed to detect the strawberry angular leaf spot pathogen Xanthomonas fragariae (Xf) and the strawberry bacterial blight pathogen Xanthomonas arboricola pv. fragariae (Xaf). The Xf PCR (Xf gyrB) was designed within regions of the gyraseB
Rena Shimizu et al.
Plant physiology, 149(2), 841-850 (2008-12-17)
The WUSCHEL-related homeobox (WOX) gene PRESSED FLOWER1 (PRS1) performs a conserved function during lateral organ development in Arabidopsis (Arabidopsis thaliana). Expressed in the periphery of the shoot meristem, PRS1 recruits founder cells that form lateral domains of vegetative and floral

Articles

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocoles

Extract-N-Amp™ Plant PCR Kits Protocol

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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