W0393

Monoclonal Anti-WRN antibody produced in mouse

clone 195C, purified from hybridoma cell culture

• Synonyme(s) : Anti-RECQ3, Anti-RECQL2, Anti-RECQL3, Anti-Werner syndrome helicase, Anti-Werner syndrome protein
• Numéro MDL: MFCD02264220
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 195C, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~167 kDa
• Espèces réactives: human
• Concentration: ~1 mg/mL
• Technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells
• Isotype: IgG1
• Numéro d'accès UniProt: Q14191
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... WRN(7486)

Description

Description générale

Anti-WRN antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 195C produced by the fusion of mouse myeloma cells (p3-NS1/Ag4-1) and splenocytes from BALB/c mice immunized with a recombinant fusion protein fragment of human WRN. Werner syndrome ATP-dependent helicase (WRN) is located on chromosome 8 and is 167 kDa. The WRN protein belongs to the RecQ helicase family of DNA helicases.

Immunogène

recombinant fusion protein fragment of human WRN (amino acids 1074-1432).

Application

Anti-WRN antibody has been used in

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunohistochemistry
• western blotting

Actions biochimiques/physiologiques

Werner syndrome ATP-dependent helicase (WRN) protein has been demonstrated to possess three known catalytic activities: 3′-5′ helicase, exonuclease and ATPase activities. The C-terminal region of the protein contains a nuclear localization signal (NLS), thus targeting WRN protein to the nucleus, nucleolus and nucleoplasmic foci. The involvement of WRN in multiple DNA metabolic process suggests its function as a tumor suppressor. Mutations in the WRN protein results in Werner syndrome (WS). The epigenetic inactivation of WRN leads to loss of WRN-exonuclease activity, resulting in increased chromosomal instability and hypersensitivity to chemotherapeutic drugs.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)