TP0200
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification
sufficient for ~50 manual assays
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produits recommandés
Description
wavelength 500-750nm
Niveau de qualité
Utilisation
sufficient for ~50 manual assays
Plage de mesure du seuil de détection de la protéine
0-1 mg/mL
Paramètres
18-26 °C temperature
200 μL sample volume
Température de stockage
2-8°C
Description générale
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Application
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
- in the stored samples of rat liver microsomal fractions
Principe
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mLFDS
- Folin & Ciocalteu’s phenol reagent 5 mL
- Protein standard 5 mL
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Code de la classe de stockage
8A - Combustible, corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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