T9822

Anti-Tubulin, Polyglutamylated antibody, Mouse monoclonal

clone B3, purified from hybridoma cell culture

• Synonyme(s) : Anti-K-ALPHA-1
• Numéro MDL: MFCD03792435
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: B3, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~50 kDa
• Espèces réactives: mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken
• Technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract
• Isotype: IgM
• Numéro d'accès UniProt: P68363
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TUBA4A(7277) , TUBB(203068) ; mouse ... Tuba1a(22142) , Tubb2b(73710) ; rat ... Tuba1a(64158)

Description

Description générale

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).

Tubulin is a cellular colchicine-binding protein.

Immunogène

purified sea urchin (Lytechinus pictus) sperm axonemal proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:

• immunoprecipitation
• immunohistochemistry
• immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Actions biochimiques/physiologiques

Microtubule plays an important role in DNA segregation.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable