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Key Documents

T5326

Sigma-Aldrich

Anti-γ-Tubulin antibody, Mouse monoclonal

enhanced validation

clone GTU-88, purified from hybridoma cell culture

Synonyme(s) :

Anti-Tubulin Antibody

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody (purified from hybridoma cell culture)

Type de produit anticorps

primary antibodies

Clone

GTU-88, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen 48 kDa

Espèces réactives

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

Conditionnement

antibody small pack of 25 μL

Validation améliorée

independent
Learn more about Antibody Enhanced Validation

Concentration

~1 mg/mL

Technique(s)

immunocytochemistry: 1-2 μg/mL using HeLa cells
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

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Description générale

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Spécificité

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Immunogène

synthetic γ-tubulin peptide, conjugated to KLH

Application

Monoclonal Anti-γ-Tubulin antibody produced in mouse has been used in following studies:
  • Western blot analysis
  • Immunohistochemistry
  • Immunocytology

Actions biochimiques/physiologiques

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Clinical and cellular features in patients with primary autosomal recessive microcephaly and a novel CDK5RAP2 mutation.
Issa L
Orphanet Journal of Rare Diseases, 8:59 (2013)
Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion.
Duldulao NA and S, Sun Z
Development, 136(23), 4033-4042 (2009)
Characterization of stress response in human retinal epithelial cells.
Giansanti V
Journal of Cellular and Molecular Medicine, 17(1), 103-115 (2013)
The gamma-tubulin gene family in humans.
Wise DO
Genomics, 67(2), 164-170 (2000)
BRCA1-dependent ubiquitination of gamma-tubulin regulates centrosome number.
Starita LM
Molecular and Cellular Biology, 24(19), 8457-8466 (2004)

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