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T2145

Sigma-Aldrich

Tyrode′s Salts

Without sodium bicarbonate, powder, suitable for cell culture

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About This Item

Code UNSPSC :
12352207

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Température de stockage

2-8°C

Catégories apparentées

Description générale

Tyrode′s Salts play an important role in tissue culture. A salt solution′s basic function is to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Application

Tyrode′s Salts has been used:
  • for seeding cardiomyocytes culture for optical based phenotyping and imaging studies
  • to incubate 3T3-L1 pre-adipocytes in calcium intracellular assays
  • as a medium for the isolation and culturing of myofiber extensor digitorum longus (EDL) muscle

Quantité

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.0 g/L sodium bicarbonate.

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Felipe Mora-Bermúdez et al.
eLife, 3 (2014-07-06)
Mitotic spindle orientation is crucial for symmetric vs asymmetric cell division and depends on astral microtubules. Here, we show that distinct subpopulations of astral microtubules exist, which have differential functions in regulating spindle orientation and division symmetry. Specifically, in polarized
Theodora Pavlidou et al.
Stem cells international, 2019, 5980465-5980465 (2019-06-30)
The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of
Tomasz Maciej Stepniewski et al.
Chemical science, 12(33), 10990-11003 (2021-09-16)
Brain functions rely on neurotransmitters that mediate communication between billions of neurons. Disruption of this communication can result in a plethora of psychiatric and neurological disorders. In this work, we combine molecular dynamics simulations, live-cell biosensor and electrophysiological assays to
Fernando M Nunez et al.
STAR protocols, 1(3), 100165-100165 (2020-12-31)
Brainstem gliomas are aggressive tumors that are more prevalent in pediatric patients. The location of these tumors makes them inoperable, and currently there is no effective treatment. Recent genomic data revealed the unique biology of these tumors. The following protocol
Nina Kudryashova et al.
PLoS computational biology, 15(3), e1006597-e1006597 (2019-03-19)
Cardiac fibrosis occurs in many forms of heart disease and is considered to be one of the main arrhythmogenic factors. Regions with a high density of fibroblasts are likely to cause blocks of wave propagation that give rise to dangerous
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Protocoles

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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