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Key Documents

SRP8024

Sigma-Aldrich

TRAIL, Soluble human

recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)

Synonyme(s) :

Apo-2L, CD253, CD339, TRAIL

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

Source biologique

human

Produit recombinant

expressed in E. coli

Étiquette/Marqueur

His tagged

Pureté

>98% (SDS-PAGE)

Forme

liquid

Poids mol.

~26 kDa

Conditionnement

pkg of 20 μg

Concentration

≥0.2 mg/mL

Technique(s)

activity assay: suitable
cell culture | mammalian: suitable
transfection: suitable

Impuretés

<0.01 EU/μg endotoxin, tested

Couleur

clear

Numéro d'accès UniProt

P50591

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... TNFSF10(8743)

Description générale

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.

Application

TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.

Actions biochimiques/physiologiques

Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).

Forme physique

Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

Autres remarques

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Intracranial AAV-sTRAIL combined with lanatoside C prolongs survival in an orthotopic xenograft mouse model of invasive glioblastoma.
Crommentuijn MH
Molecular Oncology, 10(4), 625-634 (2016)
Association between endometriosis and polymorphisms in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), TRAIL receptor and osteoprotegerin genes and their serum levels.
Kim H
Archives of Gynecology and Obstetrics, 286(1), 147-153 (2012)
Genome-Scale MicroRNA and Small Interfering RNA Screens Identify Small RNA Modulators of TRAIL-Induced Apoptosis Pathway
Dmitriy Ovcharenko
Cancer Research (2007)
Association of TRAIL and Its Receptors with Large-Artery Atherosclerotic Stroke.
Pan X
PLoS ONE, 10(9), e0136414-e0136414 (2015)
Association of Polymorphisms in TRAIL and Chronic Hepatitis B in Chinese Han Populations from Shandong Province.
Wang F
Annals of Clinical and Laboratory Science, 46(4), 374-379 (2016)
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