SRP0713

PFTK1 (CDK14)/CYCLINY, Active

PRECISIO^® Kinase, recombinant

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Pureté: 70-95% (SDS-PAGE)
• Forme: frozen liquid
• Activité spécifique: 2.0-3.5 nmol/min·mg (radiometric)
• Poids mol.: ~64 kDa (Cyclin Y); ~72 kDa (PFTK1 (CDK14))
• Concentration: 0.1 μg/μL
• Numéro d'accès: NM_012395; NM_145012
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CCNY(219771) , CDK14(5218)

Description

Application

Useful for Kinase assay and Western blot.

Actions biochimiques/physiologiques

Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PFTK1 is a member of the CDC2 - related protein kinase family which is expressed primarily in the postnatal and adult nervous system ⁽¹⁾. PFTK1 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to PFTK1 binding ⁽²⁾. PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Stockage et stabilité

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Informations légales

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable