SRP0211

PTP-D2/PTPN14 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonyme(s) : Cytoskeletal-associated protein tyrosine phosphatase, PTP36, PTPN14, Pez, Protein tyrosine phosphatase, non-receptor type 14
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 61 kDa
• Conditionnement: pkg of 20 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_005401
• Numéro d'accès UniProt: Q15678
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PTPN14(5784)

Description

Description générale

Catalytic domain, (amino acids 817-1188), (Gene bank accession number NM_005401) with N-terminal GST tag, MW = 61 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Définition de l'unité

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.