SRP0169

JMJD2B Active human

recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)

• Synonyme(s) : JHDM3B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, KIAA0876, Lysine-specific demethylase 4B (KDM4B)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥40% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 57 kDa
• Conditionnement: pkg of 100 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_015015
• Numéro d'accès UniProt: O94953
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... KDM4B(23030)

Description

Description générale

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.