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Key Documents

SRP0168

Sigma-Aldrich

EED human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonyme(s) :

HEED, Rinchik 5, WAIT1, WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in baculovirus infected insect cells

Pureté

≥90% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

51 kDa

Conditionnement

pkg of 50 μg

Concentration

>0.02 mg/mL

Numéro d'accès NCBI

NM_003797

Numéro d'accès UniProt

O75530

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... EED(8726)

Description générale

EED (ectoderm development protein) is the part of the polycomb repressive complex 2 (PRC2) where it interacts with EZH2 (enhancer of zeste homolog 2). The protein has seven WD domains (β-transducin domains). The EED gene is mapped to human chromosome 11q14.
Human Embryonic Ectoderm Development (hEED), also known as WAIT-1, GenBank Accession No. NM_003797, amino acids 2-end with N-terminal FLAG-tag, MW = 51kDa, expressed in Sf9 cells via a baculovirus expression system.

Actions biochimiques/physiologiques

EED (ectoderm development protein) is required for proper EZH2 (enhancer of zeste homolog 2)-mediated methyltransferase activity. It also associates with PRC1 (polycomb repressive complex 1) and helps in bringing PRC1 to H3K27me3 (trimethylated lysine residue at position 27 in the protein histone H3) loci. EED also increases PRC1-mediated H2A (histone 2A) ubiquitin E3 ligase activity. Mutations in the EED gene are associated with overgrowth, intellectual disability and dysmorphic features. Change in the activity of EED is linked with the pathogenesis of myeloid disorders.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

EED-associated overgrowth in a second male patient.
Cohen AS and Gibson WT
Journal of Human Genetics, 61, 831-831 (2016)
EED mutants impair polycomb repressive complex 2 in myelodysplastic syndrome and related neoplasms.
Ueda T, et al.
Leukemia, 26, 2557-2557 (2012)
The central role of EED in the orchestration of polycomb group complexes.
Cao Q, et al.na
Nature Communications, 5, 3127-3127 (2014)
Ioannis Papoulidis et al.
Molecular cytogenetics, 8, 71-71 (2015-09-22)
Interstitial deletions of the long arm of chromosome 11 are rare, and they could be assumed as non-recurrent chromosomal rearrangements due to high variability of the size and the breakpoints of the deleted region. The exact region of the deletion

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