SRP0133

EZH2/EED inactive  human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonyme(s) : ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥70% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 51 kDa (EED); 86 kDa (EZH2)
• Conditionnement: pkg of 20 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_003797 (EED); NM_004456
• Numéro d'accès UniProt: Q15910
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... EZH2(2146)

Description

Description générale

Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW= 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa, co-expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.