SRP0122

LSD1 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonyme(s) : Amine oxidase (flavin containing) domain 2 (AOF2), BRAF35-HDAC complex protein BHC110, KIAA0601, Lysine (K)-specific demethylase 1 (LSD1), Lysine-specific histone demethylase 1A (KDM1A)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥70% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 103 kDa
• Conditionnement: pkg of 50 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_015013
• Numéro d'accès UniProt: O60341
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... KDM1A(23028)

Description

Description générale

Human LSD1, AOF2, amine oxidase, flavin containing, domain 2, GenBank Accession No. NM_015013, amino acids 158-end with N-terminal ST tag, MW = 103 kDa, expressed in E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable