SRP0112

HDAC-10 human

recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)

• Synonyme(s) : HD10, Histone deacetylase 10
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥10% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 79 kDa
• Conditionnement: pkg of 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_032019
• Numéro d'accès UniProt: Q969S8
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... HDAC10(83933)

Description

Description générale

Human HDAC10, amino acids 1-481, GenBank Accession No. NM_032019, with N-terminal GST tag, MW = 79 kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.