SRE0066
Guanidine hydrochloride solution, 6M
Synonyme(s) :
Aminoformamidine hydrochloride, GuHCl, Guanidinium chloride, Guanidium chloride
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About This Item
Code UNSPSC :
12352209
Nomenclature NACRES :
NA.25
Produits recommandés
Forme
liquid
Niveau de qualité
Concentration
6 M
Application(s)
diagnostic assay manufacturing
Température de stockage
room temp
Catégories apparentées
Application
Guanidine hydrochloride is commonly used as a denaturant, because of its ability to break hydrogen bonds between amino acid residues. By breaking these bonds, the 3D conformation of the protein is unfolded and the aqueous solubility of the protein is greatly increased. Once denatured, the protein can be easily reduced, modified, or analyzed, in a variety of procedures. Further processing of the sample can allow the proteins to be analyzed by other common protein analysis methods including mass spectrometry, electrophoresis, and enzymatic digests.
Guanidine hydrochloride has also typically been used for the isolation of RNA, to denature globular proteins, and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.
Guanidine hydrochloride has also typically been used for the isolation of RNA, to denature globular proteins, and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.
Forme physique
This product is a ready-to-use 6 M guanidine hydrochloride solution. The 6 M Guanidine hydrochloride solution may be used as a stock solution to prepare solutions of lower concentrations as appropriate.
Note: If precipitation is present in the 6 M buffer, warm the bottle to 37 °C and mix until completely dissolved prior to use.
Note: If precipitation is present in the 6 M buffer, warm the bottle to 37 °C and mix until completely dissolved prior to use.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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