SAB5801050

Alpaca Anti-Chicken IgY Biotin single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonyme(s) : Recombinant Alpaca single variable domain secondary Nanobody
• Code UNSPSC : 12352203

Propriétés

• Source biologique: alpaca
• Produit recombinant: expressed in E. coli
• Conjugué: biotin
• Forme d'anticorps: affinity purified immunoglobulin
• Type de produit anticorps: secondary antibodies
• Clone: recombinant monoclonal
• Forme: lyophilized solid
• Poids mol.: 14 kDa
• Espèces réactives: Alpaca, chicken
• Technique(s): ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• Taille: 0.1 mL
• Couleur: colorless
• Isotype: VHH
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Alpaca Anti-Chicken IgY Biotin single domain Antibody is designed to specifically detect chicken IgY. It is a monovalent, single domain, recombinant antibody to chicken IgY coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Chicken IgY Biotin single domain Antibody detects the chicken IgY selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.
Hens egg yolk immunoglobulins IgY can be transferred from the serum of mother hen to the offspring egg yolk to acquire immunity. In the immunodiagnostic technologies, IgY is an excellent antibody for using in immunological assays involving mammalian sera, due to discriminative properties of IgY compared to mammalian IgG, as IgY does not react with the rheumatoid factor and human anti-mouse IgG antibodies do not activate the complement system and do not bind to Fc receptor. Also, they have poor cross reactivity to mammalian IgG due to immunological differences. IgY does not contain a hinge region but does contain an additional constant domain. The whole IgY molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgYcontains two heavy and light chains. The common IgY is monomeric with a molecular weight of approximately 170 kDa.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Spécificité

Fc region of chicken IgY
Isotype: VHH domain of alpaca IgG2b/2c

Immunogène

Recombinant Alpaca single variable domain secondary nano antibody

Conditionnement

100 μg in Glass bottle

Forme physique

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Stockage et stabilité

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Autres remarques

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317 - H412
• Conseils de prudence: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Classification des risques: Aquatic Chronic 3 - Skin Sens. 1
• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable