SAB4200879

Anti-DDX6-Alexa488 antibody, Mouse monoclonal

clone DDX6-34, purified from hybridoma cell culture

• Synonyme(s) : ATP-dependent RNA helicase p54, DEAD box protein 6, HLR2, Oncogene RCK, Probable ATP-dependent RNA helicase DDX6
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Conjugué: ALEXA FLUOR^™ 488 conjugate
• Niveau de qualité: 200
• Forme d'anticorps: purified from hybridoma cell culture
• Clone: DDX6-34
• Forme: liquid
• Espèces réactives: bovine, monkey, canine, human, mouse
• Concentration: 1.5-2 mg/mL
• Isotype: IgG1
• Numéro d'accès UniProt: P26196
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Probable ATP-dependent RNA helicase DDX6 also known as DEAD box protein 6 or Oncogene RCK/p54, is a conserved member of the DEAD-box RNA helicase family.DDX6 plays a central role in P-body assembly in mammals and its depletion prevents P-body assembly leading to P-bodies disappearance.1

Spécificité

Monoclonal Anti-DDX6 antibody specifically recognizes DDX6 from human, mouse, monkey, canine and bovine origin.

Application

The antibody may be used in Immunofluorescence techniques to detection of DDX6.

Actions biochimiques/physiologiques

DDX6 coordinates RNA and ATP helicase binding activities and binds along the mRNA. DDX6 sequence specific binding at mRNA 5′ end, activates recruitment of the decapping complex, thus coupling translational repression and mRNA degradation.1-2 DDX6 is considered to be a P-bodies marker due to its mediating role between translational repression and P-bodies formation and toits interaction with Ago2 and the RISC complex that also mediates translational silencing by miRNAs.1-4

Viruses like Hepatitis C virus (HCV) or West Nile virus (WNV) utilize the host cell DDX6 to promote virus infection by recruiting it to the viral replication sites where it is involved in stabilization of the viral genome and the shift between translation and replication.2,5-6
DDX6 was reported to be overexpressed in colorectal adenocarcinomas, gastric cancer and in most malignant cell lines.7-9 It was suggested that DDX6 acted as an upstream cancerous enhancer through RNA-binding of HER2 and FGFR2 mRNAs that positively regulates post-transcriptional processes in cancer cells.7

Forme physique

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Stockage et stabilité

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg